The strips can detect the level of human chorionic gonadotropin (HCG) as low as 25 mIU/ml ( FDA standard) just dip the test in urine for 3 seconds and read the result in 3-5 minutes (Double lines for pregnant and single line for not pregnant) Wondfo is one of the earliest high-tech biological companies focusing on rapid diagnostics and is consistently paying much attention to the R & D. we are dedicated to making the world a better place by providing people both test line and control lines indicate that you are pregnant. The color intensity of the test bands may vary due to different concentrations of HCG at different stages of ovulation. If you start testing as early as 4 days before cycle day 1, bear in mind that your body may not yet produce enough Hcg for a positive test result. Use first-morning urine for the LH pregnancy test because the concentration is the highest right after you wake up. Dedication to the customer is available to help with any questions or concerns you may have.